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BeSEC is the new tool developed by Bettersize for optimizing chromatography. It is a dual-angle light scattering detector suitable for measuring the absolute molecular weight and distribution of proteins, synthetic and natural polymers, for example. In addition, the molecular size can be determined in the form of the radius of gyration (Rg). The system can be integrated into any SEC/GPC platform and, with its two implemented photodetectors (LALS = low angle light scattering (7°), RALS = right angle light scattering (90°)), it reliably provides differently weighted absolute molecular weights (Mn, Mw, Mz, Mp) as well as polydispersity (Pd) and Rg when combined with RI or UV detectors. This results in a wide measurement range from 1000 Da to 2 GDa.
BeSEC was developed for universal integration into any third-party SEC/GPC platform. These chromatography systems are functionally divided into sample separation and data detection stages. The separation stage consists of the solvent delivery pump, the inline degasser, the autosampler (or manual injector), and the SEC columns. After separation, various detectors—usually refractive index (RI), UV-visible, and light scattering—are used in the detection stage to characterize the molecular properties of the effluent in real time.
Starting from the solvent source, the mobile phase is degassed and pumped into the separation column via a six-way injection valve. The sample components are separated according to their size and eluted into a series of measuring systems, ideally consisting of UV, RI, and light scattering detector units (BeSEC). By combining the concentration data from UV/RI with the absolute molecular weight data from the BeSEC’s 7°/90° sensors, the system obtains a complete profile of the analyte before it reaches the waste bottle.
Proteins
Polymer molecules
